Difference between revisions of "Part:BBa K5198107"

Latest revision as of 11:12, 2 October 2024

nSEAP-NanoLuc
nSEAP-NanoLuc is the NanoLuciferase (BBa_K1734004 ) with native SEAP tag (BBa_K5198010 ). Native SEAP tag is the native signal sequence of secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a continuous quantitative reporter for gene expression by repeatedly assessing the activity of certain promoter/enhancer elements in many cell samples. SEAP is contained in exosomes and is secreted into the extracellular space. nSEAP tag sequence causes extracellular secretion of proteins. NanoLuciferase is a highly-stable enzyme that functions as a luminescent reporter. Its luminscent reaction is ATP-independent and very sensitive. The small size and extreme brightness of NanoLuc makes it an ideal fusion partner. When attached to the nSEAP tag, this nSEAP-NanoLuc signal peptide can be used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 260
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 571
Illegal NgoMIV site found at 1540
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 901
Illegal BsaI.rc site found at 1390

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 nSEAP-NanoLuc is the NanoLuciferase (<a href="https://parts.igem.org/Part:BBa_K1734004">BBa_K1734004</a>
 ) with native SEAP tag (<a href="https://parts.igem.org/Part:BBa_K5198010">BBa_K5198010</a>
-). Native SEAP tag is the native signal sequence of secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a continuous quantitative reporter for gene expression by repeatedly assessing the activity of certain promoter/enhancer elements in many cell samples. SEAP is contained in exosomes and is secreted into the extracellular space. nSEAP tag sequence causes extacellular secretion of proteins. NanoLuciferase is a highly-stable enzyme that functions as a luminescent reporter. Its luminesecnt reaction is ATP-independent and very sensitive. The small size and extreme brightness of NanoLuc makes it an ideal fusion partner. When attached to the nSEAP tag, this nSEAP-NanoLuc signal peptide can be used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.
+). Native SEAP tag is the native signal sequence of secreted embryonic alkaline phosphatase (SEAP). SEAP acts as a continuous quantitative reporter for gene expression by repeatedly assessing the activity of certain promoter/enhancer elements in many cell samples. SEAP is contained in exosomes and is secreted into the extracellular space. nSEAP tag sequence causes extracellular secretion of proteins. NanoLuciferase is a highly-stable enzyme that functions as a luminescent reporter. Its luminscent reaction is ATP-independent and very sensitive. The small size and extreme brightness of NanoLuc makes it an ideal fusion partner. When attached to the nSEAP tag, this nSEAP-NanoLuc signal peptide can be used in solubility studies to find the optimal secretion signal peptide for neoantigens in ATLAS.
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