Difference between revisions of "Part:BBa K5117042"
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<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html></li> | <li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html></li> | ||
| − | <li><i>bglB</i> gene of <i>Paenibacillus polymyxa</i> encoding a β-glucosidase (EC 3.2.1.21)</li> | + | <li><i>bglB</i> gene of <i>Paenibacillus polymyxa</i> encoding a β-glucosidase (EC 3.2.1.21), </li> |
<div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> <html><a href="https://parts.igem.org/Part:BBa_K5117030">(BBa_K5117030)</a></html></div> | <div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> <html><a href="https://parts.igem.org/Part:BBa_K5117030">(BBa_K5117030)</a></html></div> | ||
Revision as of 16:03, 1 October 2024
PcotYZ-BsRBS-PpBglB-L2-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
- ribosome binding site of Bacillus subtilis (BBa_K5117000)
- bglB gene of Paenibacillus polymyxa encoding a β-glucosidase (EC 3.2.1.21),
- cotY gene of Bacillus subtilis (BBa_K5117022)
- bidirectional terminator B0014 (BBa_B0014)
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117030)
Biosafety level: S1
Target organism: Bacillus subtilis
Main purpose of use: Immobilization of PpBglB on the spore crust of B. subtilis (spore surface display)
Application: Degradation of cellobiose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1627
- 1000COMPATIBLE WITH RFC[1000]