Difference between revisions of "Part:BBa K5117039"
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<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html>.</li>
 
<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html>.</li>
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</ul>
  
  

Revision as of 14:47, 1 October 2024


PcotYZ-BsRBS-BhBglA-L2-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • bglA gene of Bacillus halodurans encoding a beta-glucosidase (EC 3.2.1.21),
    • addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117027),
  • cotY gene of Bacillus subtilis (BBa_K5117022),
  • bidirectional terminator B0014 (BBa_B0014).


Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Immobilization of BhBglA on the spore crust of B. subtilis (spore surface display)

Application: Degradation of cellobiose


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 764
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1624
  • 1000
    COMPATIBLE WITH RFC[1000]