Difference between revisions of "Part:BBa K5117045"
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This part serves as transcriptional unit composed of:
 
This part serves as transcriptional unit composed of:
  
−
- promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021),
+
<ul>
 +
<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021)</a></html></li>
  
−
- ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000),
+
<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html></li>
  
−
- gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), a homologue of the leaf-branch compost cutinase (LCC), codon optimized for <i>Bacillus subtilis</i> (Xi <i>et al.</i> 2021) without any signal peptide,
+
<li>gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101), </li>
  
−
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> (BBa_K5117033),
+
<div style="text-indent: 1px;">a homologue of the leaf-branch compost cutinase (LCC), codon optimized for <i>Bacillus subtilis</i> (Xi <i>et al.</i> 2021) without any signal peptide,</div>
  
−
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),
+
<div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> <html><a href="https://parts.igem.org/Part:BBa_K5117033">(BBa_K5117033)</a></html></div>
  
−
- bidirectional terminator B0014 (BBa_B0014).
+
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html></li>
  
 +
<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html></li>
 +
</ul>
  
  
−
<!-- Add more about the biology of this part here
+
<b>Biosafety level:</b> S1
−
===Usage and Biology===
+
 
 +
<b>Target organism:</b> <i>Bacillus subtilis</i>
 +
 
 +
<b>Main purpose of use:</b> Immobilization of BhrPET on the spore crust of <i>B. subtilis</i> (spore surface display)
 +
 
 +
<b>Application:</b> Degradation of PET
 +
 
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5117045 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5117045 SequenceAndFeatures</partinfo>

Revision as of 16:33, 1 October 2024


PcotYZ-BsRBS-BhrPET-L2-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021)
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • gene of the uncultured bacterium HR29 encoding a polyethylene terephthalate hydrolase (PETase, EC 3.1.1.101),
    • a homologue of the leaf-branch compost cutinase (LCC), codon optimized for Bacillus subtilis (Xi et al. 2021) without any signal peptide,
    addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117033)
  • cotY gene of Bacillus subtilis (BBa_K5117022)
  • bidirectional terminator B0014 (BBa_B0014)


Biosafety level: S1

Target organism: Bacillus subtilis

Main purpose of use: Immobilization of BhrPET on the spore crust of B. subtilis (spore surface display)

Application: Degradation of PET


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1063
    Illegal AgeI site found at 695
    Illegal AgeI site found at 740
  • 1000
    COMPATIBLE WITH RFC[1000]