Difference between revisions of "Part:BBa K5117040"
| Line 12: | Line 12: | ||
<li><i>bglA</i> gene of <i>Bacillus halodurans</i> encoding a beta-glucosidase (EC 3.2.1.21),</li> | <li><i>bglA</i> gene of <i>Bacillus halodurans</i> encoding a beta-glucosidase (EC 3.2.1.21),</li> | ||
| − | <div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG <html><a href="https://parts.igem.org/Part:BBa_K5117028">(BBa_K5117028),</div> | + | <div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG <html><a href="https://parts.igem.org/Part:BBa_K5117028">(BBa_K5117028)</a></html>,</div> |
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html>,</li> | <li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html>,</li> | ||
Revision as of 12:30, 1 October 2024
PcotYZ-BsRBS-BhBglA-L3-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
- ribosome binding site of Bacillus subtilis (BBa_K5117000),
- bglA gene of Bacillus halodurans encoding a beta-glucosidase (EC 3.2.1.21),
- cotY gene of Bacillus subtilis (BBa_K5117022),
- bidirectional terminator B0014 (BBa_B0014).
addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117028),
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 764
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1597
- 1000COMPATIBLE WITH RFC[1000]