Difference between revisions of "Part:BBa K5117039"
Line 12: Line 12:
 
<li><i>bglA</i> gene of <i>Bacillus halodurans</i> encoding a beta-glucosidase (EC 3.2.1.21),</li>
 
<li><i>bglA</i> gene of <i>Bacillus halodurans</i> encoding a beta-glucosidase (EC 3.2.1.21),</li>
  
−
<div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 <html><a href="https://parts.igem.org/Part:BBa_K5117027">(BBa_K5117027)</a></html>,</div>
+
<div style="text-indent: 1px;">addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)<sub>4</sub> <html><a href="https://parts.igem.org/Part:BBa_K5117027">(BBa_K5117027)</a></html>,</div>
  
 
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html>,</li>
 
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html>,</li>

Revision as of 12:08, 1 October 2024


PcotYZ-BsRBS-BhBglA-L2-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • bglA gene of Bacillus halodurans encoding a beta-glucosidase (EC 3.2.1.21),
    • addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117027),
  • cotY gene of Bacillus subtilis (BBa_K5117022),
  • bidirectional terminator B0014 (BBa_B0014).


    • Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 764
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1624
    • 1000
      COMPATIBLE WITH RFC[1000]