Difference between revisions of "Part:BBa K5115085"
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__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| − | This part is FKBP with F36V mutation, which is a signal transducer. Binding with F1v, the target protein can get dimerized with B/B Homodimerizer in the culture solution. | + | This part is FKBP with F36V mutation, which is a signal transducer. Binding with F1v, the target protein can get dimerized with B/B Homodimerizer in the culture solution. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | In our experiment, we adopted the iDimerize kit, to learn fully about this kit, please check [https://www.takarabio.com/documents/User%20Manual/iDimerize%20Inducible%20Homodimer%20System%20User%20Manual%20%28PT5178/iDimerize%20Inducible%20Homodimer%20System%20User%20Manual%20%28PT5178-1%29_111115.pdf?srsltid=AfmBOoqcK3gD-UsdZMD_JLop5oY-5L8W9a82H6GI4TmsKvN2gEBf37iH iDimerize™ Inducible Homodimer System User Manual] | ||
| + | |||
In [https://parts.igem.org/Part:BBa_K5115086 BBa_K5115086(NixA-F1v)] and [https://parts.igem.org/Part:BBa_K5115087 BBa_K5115087(F1v-NixA)], we link this part with [https://parts.igem.org/Part:BBa_K5115071 BBa_K5115071(NixA)] in two different ways to find the more efficient way of dimerizing the NixA protein. | In [https://parts.igem.org/Part:BBa_K5115086 BBa_K5115086(NixA-F1v)] and [https://parts.igem.org/Part:BBa_K5115087 BBa_K5115087(F1v-NixA)], we link this part with [https://parts.igem.org/Part:BBa_K5115071 BBa_K5115071(NixA)] in two different ways to find the more efficient way of dimerizing the NixA protein. | ||
Revision as of 11:08, 29 September 2024
F1v (FKBP with F36V mutation) without stop codon
Introduction
This part is FKBP with F36V mutation, which is a signal transducer. Binding with F1v, the target protein can get dimerized with B/B Homodimerizer in the culture solution.
Usage and Biology
In our experiment, we adopted the iDimerize kit, to learn fully about this kit, please check iDimerize™ Inducible Homodimer System User Manual
In BBa_K5115086(NixA-F1v) and BBa_K5115087(F1v-NixA), we link this part with BBa_K5115071(NixA) in two different ways to find the more efficient way of dimerizing the NixA protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]