Difference between revisions of "Part:BBa J23109:Experience"

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We used a number of the constitutive promoter family members for testing our biobricks. The constitutive promoters show the expected level of fluorescence when transformed into E. coli TOP10 cells. Placing parts behind the promoters turned out to be relatively straight forward. We used this part in combination with several biobricks for building our constructs e.g. <partinfo>BBa_I750016</partinfo> and <partinfo>BBa_K190028</partinfo> were placed behind the promoters.
 
We used a number of the constitutive promoter family members for testing our biobricks. The constitutive promoters show the expected level of fluorescence when transformed into E. coli TOP10 cells. Placing parts behind the promoters turned out to be relatively straight forward. We used this part in combination with several biobricks for building our constructs e.g. <partinfo>BBa_I750016</partinfo> and <partinfo>BBa_K190028</partinfo> were placed behind the promoters.
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<partinfo>BBa_J23106 AddReview 5</partinfo>
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<I>iGEM HKU 2011</I>
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To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain.
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The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression.
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|[[Image:MRFP data graph.png|450px|thumb|center|mRFP fluorescence intensity under different promoters]]||
 
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<!-- DON'T DELETE --><partinfo>BBa_J23109 EndReviews</partinfo>
 
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Revision as of 14:07, 5 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J23109

BBa_K190025 (Planning) constitutive promoter with GVP cluster

BBa_K190031 (Planning) constitutive promoter with fMT

User Reviews

UNIQ3769a2e6970065bb-partinfo-00000000-QINU

•••••

iGEM Groningen 2009

We used a number of the constitutive promoter family members for testing our biobricks. The constitutive promoters show the expected level of fluorescence when transformed into E. coli TOP10 cells. Placing parts behind the promoters turned out to be relatively straight forward. We used this part in combination with several biobricks for building our constructs e.g. BBa_I750016 and BBa_K190028 were placed behind the promoters.

•••••

iGEM HKU 2011

To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain. The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression.

mRFP fluorescence intensity under different promoters

UNIQ3769a2e6970065bb-partinfo-00000005-QINU