Composite

Part:BBa_K190025

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-08-25)


GVP with low constitutive promoter

The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the constitutive promoter BBa_J23109 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
    Illegal BamHI site found at 4182
    Illegal XhoI site found at 3870
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 828
    Illegal NgoMIV site found at 1230
    Illegal AgeI site found at 4149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2640
    Illegal SapI.rc site found at 3796


[edit]
Categories
Parameters
None