Difference between revisions of "Part:BBa K5117040"

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This part serves as transcriptional unit composed of:
 
This part serves as transcriptional unit composed of:
  
- promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021),  
+
<ul>
 +
<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117021">(BBa_K5117021)</a></html>, </li>
  
- ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000),  
+
<li>ribosome binding site of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117000">(BBa_K5117000)</a></html>, </li>
  
- <i>bglA</i> gene of <i>Bacillus halodurans</i> encoding a beta-glucosidase (EC 3.2.1.21),
+
<li><i>bglA</i> gene of <i>Bacillus halodurans</i> encoding a beta-glucosidase (EC 3.2.1.21),</li>
  
addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117028),
+
<div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG <html><a href="https://parts.igem.org/Part:BBa_K5117028">(BBa_K5117028),</div>
  
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),
+
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> <html><a href="https://parts.igem.org/Part:BBa_K5117022">(BBa_K5117022)</a></html>,</li>
 
+
- bidirectional terminator B0014 (BBa_B0014).
+
  
 +
<li>bidirectional terminator B0014 <html><a href="https://parts.igem.org/Part:BBa_B0014">(BBa_B0014)</a></html>.</li>
 +
</ul>
  
  

Revision as of 12:27, 1 October 2024


PcotYZ-BsRBS-BhBglA-L3-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
  • ribosome binding site of Bacillus subtilis (BBa_K5117000),
  • bglA gene of Bacillus halodurans encoding a beta-glucosidase (EC 3.2.1.21),
  • addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117028),
  • cotY gene of Bacillus subtilis (BBa_K5117022),</li>
  • bidirectional terminator B0014 (BBa_B0014).
  • </ul>


    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 764
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1597
    • 1000
      COMPATIBLE WITH RFC[1000]