Difference between revisions of "Part:BBa K5143015"
Line 35: Line 35:
 
         <div class="image-caption">Venus Yellow Fluorescent Protein used as a transcriptional reporter.</div>
 
         <div class="image-caption">Venus Yellow Fluorescent Protein used as a transcriptional reporter.</div>
 
     </div>
 
     </div>
 +
<br>
 
     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
     <p>
 
     <p>
−
       The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in <I>Saccharomyces cerevisiae</i>. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (<a href="https://parts.igem.org/Part:BBa_K5143009">BBa_K5143009</a>) in 5'. See the composite part here:
+
       The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in <I>Saccharomyces cerevisiae</i>. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (<a href="https://parts.igem.org/Part:BBa_K5143009">BBa_K5143009</a>) in 5'. See the composite part here: <a href="https://parts.igem.org/Part:BBa_K5143026" target="_blank">BBa_K5143026</a>
−
    </p>
+
     <br>
−
    <h1>References</h1>
+
−
     <p>
+
−
1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).<br>
+
−
2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
+
−
    </p>
+
 
</body>
 
</body>
 
</html>
 
</html>
−
 
−
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
 
<!-- -->
 
<!-- -->
 
<h1>Sequence and Features</h1>
 
<h1>Sequence and Features</h1>
 
<partinfo>BBa_K5143015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5143015 SequenceAndFeatures</partinfo>
−
 
+
br>
 +
</p>
 +
    <h1>References</h1>
 +
    <p>
 +
1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).<br>
 +
<br>
 +
2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).
 +
    </p>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 14:22, 1 October 2024


Venus : reporter Yellow Fluorescent Protein

Protein Description

Description

Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm.

Venus
Venus Yellow Fluorescent Protein used as a transcriptional reporter.

Construction

The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (BBa_K5143009) in 5'. See the composite part here: BBa_K5143026

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

br> </p>

References

1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).

2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).