Difference between revisions of "Part:BBa K5349005:Design"

 
(→‎Source)
Line 15: Line 15:
 
UL23 : <a href="https://parts.igem.org/Part:BBa_K5349000:Design" target="_blank">UL23: Refer to K5349000</a>
 
UL23 : <a href="https://parts.igem.org/Part:BBa_K5349000:Design" target="_blank">UL23: Refer to K5349000</a>
  
−
sfGFP : <a href="https://parts.igem.org/Part:BBa_K4387001:Design" target="_blank">sfGFP: Refer to K4387001</a>
+
sfGFP : <a href="https://parts.igem.org/Part:BBa_K2553008:Design" target="_blank">sfGFP: Refer to K2553008</a>
−
 
+
  
 
===References===
 
===References===

Revision as of 06:30, 26 September 2024


UL23-sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1611
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 879
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Twist Bioscience's DNA synthesis service


Source

UL23 : <a href="https://parts.igem.org/Part:BBa_K5349000:Design" target="_blank">UL23: Refer to K5349000</a>

sfGFP : <a href="https://parts.igem.org/Part:BBa_K2553008:Design" target="_blank">sfGFP: Refer to K2553008</a>

References