Difference between revisions of "Part:BBa K5205001"
 
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===Usage and Biology===
 
===Usage and Biology===
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The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) (BBa_K5205002) to form the Non-OmpA system (BBa_K5205003). This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).
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The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) [https://parts.igem.org/Part:BBa_K5205002 BBa_K5205002] to form the Non-OmpA system [https://parts.igem.org/Part:BBa_K5205003 BBa_K5205003]. This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).
  
  
 
==References==
 
==References==
 
Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0
 
Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0

Latest revision as of 02:46, 24 September 2024


Non, signal-like sequence

The Non sequence is an artificially designed signal-like sequence that directs newly synthesized proteins to the cell membrane via a non-classical secretion pathway (Jeiranikhameneh et al., 2017).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

The Non signal-like sequence always combined with OmpA a part of the outer membrane protein A (OmpA) BBa_K5205002 to form the Non-OmpA system BBa_K5205003. This system efficiently displays fusion proteins on the cell surface in E. coli (Jeiranikhameneh et al., 2017).


References

Jeiranikhameneh, M., Razavi, M. R., Irani, S., Siadat, S. D., & Oloomi, M. (2017). Designing novel construction for cell surface display of protein E on Escherichia coli using non-classical pathway based on Lpp-OmpA. AMB Express, 7(1), 53. https://doi.org/10.1186/s13568-017-0350-0