Difference between revisions of "Part:BBa K5198110"

 
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<partinfo>BBa_K5198110 short</partinfo>
 
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CTR-sfGFP is composed of the Human Trypsinogen-2 protein signal sequence (<a href="https://parts.igem.org/Part:BBa_K5198011">BBa_K5198011</a>
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) with sfGFP (<a href="https://parts.igem.org/Part:BBa_K5198012">BBa_K5198012</a>
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), Superfolder green fluorescent protein. CTR is the proenzyme precursor to trypsin, and is secreted from the pancreas in this precursor form to later aid in digestion. At 51 bp long, the CTR signal sequence is relatively small and should not have significant effects on the protein attached. In the case of CTR-sfGFP, this attached protein is superfolder GFP.
  
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<img src="https://static.igem.wiki/teams/5198/results/figure-2v3.webp" alt="Description of the image" style="width: 400px; height: auto;">
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<p><b>Figure 2.</b> Fluorescence readings of sfGFP with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is sfGFP fused with an HA tag, which does not exhibit extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background fluorescence. </p>
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Revision as of 12:03, 2 October 2024


CTR-sfGFP
CTR-sfGFP is composed of the Human Trypsinogen-2 protein signal sequence (BBa_K5198011 ) with sfGFP (BBa_K5198012 ), Superfolder green fluorescent protein. CTR is the proenzyme precursor to trypsin, and is secreted from the pancreas in this precursor form to later aid in digestion. At 51 bp long, the CTR signal sequence is relatively small and should not have significant effects on the protein attached. In the case of CTR-sfGFP, this attached protein is superfolder GFP.

Description of the image

Figure 2. Fluorescence readings of sfGFP with different signal peptide fusions in HEK293T cell supernatant. The negative control (NC) is sfGFP fused with an HA tag, which does not exhibit extracellular secretion. Error bars represent the standard deviation (SD) of 4 biological replicates. The data was normalized by subtracting background fluorescence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 618
  • 1000
    COMPATIBLE WITH RFC[1000]