Difference between revisions of "Part:BBa K4883005"

 
 
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Insert a 2A self-cleaving peptide, ERBV-1, between RIB1 and RIB7. ERBV-1 helps to achieve bicistronic gene expression in Saccharomyces cerevisiae.
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This is a riboflavin synthase RIB1, connected with an ERBV-1 2A self-cleaving peptide, followed by another riboflavin synthase RIB7.  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
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RIB1 ([https://parts.igem.org/Part:BBa_K4883001 BBa_K4883001]) and RIB7 ([https://parts.igem.org/Part:BBa_K4883002 BBa_K4883002]) catalyze the first and second steps of the riboflavin (vitamin B2) biosynthesis pathway in Saccharomyces cerevisiae. Co-overexpression of RIB1 and RIB7 could lead to overproduction of vitamin B2 in S. cerevisiae.
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For the two genes to be expressed separately and normally, the genes are divided by a 2A self-cleaving peptide, ERBV-1, which cleaves a longer peptide into two shorter peptides. ERBV-1 has been proven to have the highest cleavage efficiency in S. cerevisiae (Souza-Moreira et al., 2018).
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==References==
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Souza-Moreira TM, Navarrete C, Chen X, Zanelli CF, Valentini SR, Furlan M, Nielsen J, Krivoruchko A. Screening of 2A peptides for polycistronic gene expression in yeast. FEMS Yeast Res. 2018 Aug 1;18(5). doi: 10.1093/femsyr/foy036. PMID: 29617770.
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Latest revision as of 01:55, 6 October 2023


RIB1-ERBV-1-RIB7

This is a riboflavin synthase RIB1, connected with an ERBV-1 2A self-cleaving peptide, followed by another riboflavin synthase RIB7.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 428
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1267



Usage and Biology

RIB1 (BBa_K4883001) and RIB7 (BBa_K4883002) catalyze the first and second steps of the riboflavin (vitamin B2) biosynthesis pathway in Saccharomyces cerevisiae. Co-overexpression of RIB1 and RIB7 could lead to overproduction of vitamin B2 in S. cerevisiae.

For the two genes to be expressed separately and normally, the genes are divided by a 2A self-cleaving peptide, ERBV-1, which cleaves a longer peptide into two shorter peptides. ERBV-1 has been proven to have the highest cleavage efficiency in S. cerevisiae (Souza-Moreira et al., 2018).

References

Souza-Moreira TM, Navarrete C, Chen X, Zanelli CF, Valentini SR, Furlan M, Nielsen J, Krivoruchko A. Screening of 2A peptides for polycistronic gene expression in yeast. FEMS Yeast Res. 2018 Aug 1;18(5). doi: 10.1093/femsyr/foy036. PMID: 29617770.