Coding

Part:BBa_K4883002

Designed by: Yuqi Fu   Group: iGEM23_Hangzhou-BioX   (2023-06-29)


RIB7, GTP cyclohydrolase II from Saccharomyces cerevisiae

RIB7 encodes for diaminohydroxyphoshoribosylaminopyrimidine deaminase in S. cerevisiae. This enzyme catalyzes the second step of the riboflavin biosynthesis pathway, transforming 2,5-diamino-6-(5-phospho-D-ribosylamino)-pyrimidin-4(3H)-one (DARPP) to 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5 -phosphate (DArPP) (Gudipati et al., 2014).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 240
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 172



Usage and Biology

RIB7 is a riboflavin synthase. Overexpression of RIB7 could lead to overproduction of vitamin B2 in S. cerevisiae.

Figure 1. Gel electrophoresis of colony PCR products for verification of correct transformation of plasmid pCEV-G4-RIB7-Km into E. coli DH5α.

References

Gudipati, V.; Koch, K.; Lienhart, W.-D.; Macheroux, P. The Flavoproteome of the Yeast Saccharomyces Cerevisiae. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 2014, 1844 (3), 535–544. DOI:10.1016/j.bbapap.2013.12.015.

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