Difference between revisions of "Part:BBa K4491004"
Line 50: Line 50:
 
|-
 
|-
 
|'''Submitted by'''
 
|'''Submitted by'''
−
|[]
+
|[https://2022.igem.wiki/cambridge/index.html Cambridge iGEM 2022]
 
|}
 
|}
  

Revision as of 13:55, 10 October 2022


Terminator.png

DT54

  • ultra strong double terminator characterised in the paper Precision design of stable genetic circuits carried in highly-insulated E. coli genomic landing pads (Park et al, 2018). :)


  • Double terminator allows transcription termination in both directions. This is important as during the codon optimisation process and when building genetic circuits, there is a chance that a reverse promoter can be produced somewhere along the genetic circuits.

Results characterised by Park et al., 2018

  • Terminator strength (forward): 1800
  • Terminator strength (reverse): 30


E-cadherin (Preproprotein, Mus Musculus)
Function Termination
Use in Bacteria
Chassis Tested E. coli
Abstraction Hierarchy Part
Related Device
RFC standard RFC10 & RFC12 & RFC21 & RFC23 & RFC25 & RFC1000 compatible
Backbone pJUMP18-Uac
Submitted by Cambridge iGEM 2022


Secondary Structure

File:Mfold-K4491004-1.png


Usage and Biology

DNA Sequence

Measurement

  • [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]