Difference between revisions of "Part:BBa K4214000"
Line 3: Line 3:
  
  
−
This part codes for human Gastric Intrinsic Factor. We used the human sequence of the gene.
+
This part codes for human Gastric Intrinsic Factor protein (AA19-417). The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.  
−
The gene is located in chromosome 11q12.1. This gene is a part of the cobalamin transporters group.  
+
 
−
It is essential for the absorption of vitamin B12.  
+
We '''removed''' the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with '''part BBa_K4214001''', which '''includes''' the signal peptide sequence (AA1-18).
−
Mutations in this gene may lead to pernicious anemia.
+
 
  
  
Line 12: Line 12:
  
 
*NCBI page of the human gastric intrinsic factor [https://www.ncbi.nlm.nih.gov/gene/2694]
 
*NCBI page of the human gastric intrinsic factor [https://www.ncbi.nlm.nih.gov/gene/2694]
−
*We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia
+
*We designed this biobrick in order to have a sequence for our other parts
−
*We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli. These parts are BBa_K4214001 [https://parts.igem.org/Part:BBa_K4214001] , BBa_K4214002 [https://parts.igem.org/Part:BBa_K4214002], BBa_K4214003 [https://parts.igem.org/Part:BBa_K4214003], BBa_K4214004 [https://parts.igem.org/Part:BBa_K4214004].
+
*This part of hGIF should be mentioned that does not contain the signal peptide
−
*This part of hGIF should be mentioned that does not contain the signal peptide  
+
  
  

Revision as of 08:11, 9 October 2022

Human Gastric Intrinsic Factor (hGIF)


This part codes for human Gastric Intrinsic Factor protein (AA19-417). The gene is located in chromosome 11q12.1. This gene is a part of cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.

We removed the native signal peptide (AA1-18) from the N-terminal in order to obtain a sequence that can be worked with more easily in bacterial systems. However, since we used bacterial and mammalian expression vectors in parallel, we needed to have compatible parts and decided to work only with part BBa_K4214001, which includes the signal peptide sequence (AA1-18).


Usage and Biology

  • NCBI page of the human gastric intrinsic factor [1]
  • We designed this biobrick in order to have a sequence for our other parts
  • This part of hGIF should be mentioned that does not contain the signal peptide


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 265
    Illegal BsaI.rc site found at 945
    Illegal SapI site found at 935