Difference between revisions of "Part:BBa K4197012"

Revision as of 17:23, 8 October 2022

mRFP1 under control of ihfB800 promoter

Red fluorescent protein mRFP1 expressed with a constitutive promoter of E. coli.

Introduction

This part is composed of the gene coding for the mRFP1 (given by Manon Barthe, a researcher at Toulouse Biotechnology Institute), a red fluorescent protein. It also contains the gene coding for the 800 first bp of the ihfB promoter, which was used to express mTagBFP. This promoter has been identified as a constitutive E. coli promoter (Weglenska et al., 1996). It is often used by researchers of the Toulouse Biotechnology Institute to express recombinant fluorescent proteins in E. coli (Barthe et al., 2020), as it is strong enough to allow correct expression and weak enough to avoid inclusion bodies.

Construction

The objective of the INSA-UPS 2022 team was to use ihfB800 promoter to express mRFP1 into E. coli Tuner (DE3) cells. Unfortunately, the expression of the fluorescent protein could not be achieved. (see BBa_K4197003, BBa_K4197004, and BBa_K4197005).

References

More information about the project for which the part was created: DAISY (INSA-UPS 2022)

Other parts of fluorescent proteins with ihfB800:
- mTagBFP
- mScarlet-I

1. Wȩgleńska, A., Jacob, B., & Sirko, A. (1996). Transcriptional pattern of Escherichia coli ihfB (himD) gene expression. Gene, 181(1-2), 85–88. https://doi.org/10.1016/s0378-1119(96)00468-4
2. Barthe, M., Tchouanti, J., Gomes, P. H., Bideaux, C., Lestrade, D., Graham, C., Steyer, J.-P., Meleard, S., Harmand, J., Gorret, N., Cocaign-Bousquet, M., & Enjalbert, B. (2020). Availability of the Molecular Switch XylR Controls Phenotypic Heterogeneity and Lag Duration during Escherichia coli Adaptation from Glucose to Xylose. mBio, 11(6), Article e02938-20. https://doi.org/10.1128/mbio.02938-20

Sequence and Features