Difference between revisions of "Part:BBa K4115040"
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Revision as of 11:38, 3 October 2022
J23101-B0034-LuxI-B0015
| J23101-B0034-LuxI-B0015 | |
|---|---|
| Function | gene regulator |
| Use in | E.coli |
| RFC standard | RFC 10, RFC 10 compatible |
| Backbone | pBBR1 |
| Submitted by | ShanghaiTech_China |
This composite part is used to produce 3OC6 HSL by LacI (BBa_C0161) and send it to the receiver protein LuxR (BBa_C0062)in the other E.coli.When the LuxR receives the signal, it will regulate and express the downstream report gene sfGFP, thus we can detect whether LuxI is functional by observing the fluorescence intensity under UV light.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]