Difference between revisions of "Part:BBa K4115044"

Revision as of 06:13, 3 October 2022

J23101-B0034-tagRFP-B0015

CMV:Ha-NLS-dCas9-Linker-VP16-NLS:BGH
Function	gene reporter
Use in	Prokaryotic cells
RFC standard	RFC 10, RFC 10 compatible
Backbone	pBBR1
Submitted by	[http://2022.igem.org/Team:ShanghaiTech_China]

This composite part is used to express the tagRFP(BBa_K4115013) by a constitutive promoter J23101(J23101), thus we use red fluorescence as a detection.

Proof of function

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 695
Illegal SapI.rc site found at 77

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4115044 short</partinfo>
+{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
+! colspan="2" style="background:#FFBF00;"|CMV:Ha-NLS-dCas9-Linker-VP16-NLS:BGH
+|-
+|'''Function'''
+|gene reporter
+|-
+|'''Use in'''
+|Prokaryotic cells
+|-
+|'''RFC standard'''
+|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10], RFC 10 compatible
+|-
+|'''Backbone'''
+|pBBR1<br>
+|-
+|'''Submitted by'''
+|[http://2022.igem.org/Team:ShanghaiTech_China]
+|}
 This composite part is used to express the tagRFP[https://parts.igem.org/Part:BBa_K4115013 (BBa_K4115013)] by a constitutive promoter J23101[https://parts.igem.org/Part:BBa_J23101 (J23101)], thus we use red fluorescence as a detection.
 ==Proof of function==
 <!-- Add more about the biology of this part here
 ===Usage and Biology===