Difference between revisions of "Part:BBa T2021"
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This is a VSV-G epitope tag tail domain designed to be at the C-terminus of a protein. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins. | This is a VSV-G epitope tag tail domain designed to be at the C-terminus of a protein. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The VSV-G epitope tag is derived from the C-terminal residues of vesicular stomatitis virus glycoprotein (VSV-G). Proteins can be purified or quantitatively analyzed using a monoclonal antibody to the VSV-G tag. Elution from the antibody occurs at low pH. | ||
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| + | The VSV-G is probably best used at the C-terminus of a protein of interest, but has also been used internally <cite>Battini</cite>. | ||
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Latest revision as of 15:48, 3 September 2009
VSV-G epitope tag tail domain (YTDIEMNRLGK)
This is a VSV-G epitope tag tail domain designed to be at the C-terminus of a protein. VSV-G tags are a type of epitope tag that can be used to measure the quantity of proteins.
Usage and Biology
The VSV-G epitope tag is derived from the C-terminal residues of vesicular stomatitis virus glycoprotein (VSV-G). Proteins can be purified or quantitatively analyzed using a monoclonal antibody to the VSV-G tag. Elution from the antibody occurs at low pH.
The VSV-G is probably best used at the C-terminus of a protein of interest, but has also been used internally Battini.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]