Difference between revisions of "Part:BBa K182005:Design"

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===Source===
 
===Source===
  
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href="https://parts.igem.org/Part:BBa_K182004">BBa_K182004</a></html> was used as the upstream part,  <html><a href="https://parts.igem.org/Part:BBa_S03518">BBa_S03518</a></html> was used as the downstream part.  
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<html><a href="https://parts.igem.org/Part:BBa_K182004">BBa_K182004</a></html> was used as the upstream part,  <html><a href="https://parts.igem.org/Part:BBa_S03518">BBa_S03518</a></html> was used as the downstream part.  
 
<html><a href="https://parts.igem.org/Part:pSB1AC3">pSB1AC3</a></html> was our recipient vector.
 
<html><a href="https://parts.igem.org/Part:pSB1AC3">pSB1AC3</a></html> was our recipient vector.
  
 
===References===
 
===References===

Revision as of 13:09, 21 August 2009

TetR regulated by CI operator (RBS+, Term-) with LVA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Laci cloning step 1Aberdeen2009.png

The cloning involved ligating K182004 (upstream) and S03518 (downstream) together into a Cholramphenicol vector, creating the BioBrick named K182005. K182005 was then tested using BB_I13600, by transforming them both into a cell and observing the behaviour of the CFP expression.

Source

BBa_K182004 was used as the upstream part, BBa_S03518 was used as the downstream part. pSB1AC3 was our recipient vector.

References