Difference between revisions of "Part:BBa T2012:Design"
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Latest revision as of 20:29, 13 August 2009
Strep-tag tail domain (WRHPQFGG)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This domain encodes the Strep-tag sequence WRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.
The Strep-tag was originally isolated as a nine amino acid peptide AWRHPQFGG. However, based on the literature, it appears that the N-terminal alanine is not necessary for function so it is not included here.
Source
This is a synthetic sequence.
References
Not a comprehensive list
<biblio>
- Schmidt93 pmid=8433964
// this looks to be the original reference for the Strep-tag
- Skerra96 pmid=8636976
// original reference for Strep-tag II
- Voss97 pmid=9415448
// original reference for streptavidin mutant with enhanced affinity for Strep-tag and Strep-tag II. The variant was later called StrepTactin.
- Skerra99 pmid=10796988
- Skerra00 pmid=11036648
// history of Strep-tag system
- Skerra07 pmid=17571060
// includes brief overview of Strep-tag system </biblio>