Difference between revisions of "Part:BBa K4143340"
Line 7: | Line 7: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | |||
+ | [[File:Experiment-construct.png|400px]] | ||
+ | |||
+ | |||
<!-- --> | <!-- --> |
Revision as of 20:50, 27 September 2022
AMP Encapsulin Composite
This composite part includes an antimicrobial peptide (HBCM2), TEV protease site, targeting peptide, and T4GALA encapsulin. The encapsulin is a protein nanocompartment that sequesters cargo carrying a targeting peptide. In this case, the antimicrobial peptide (AMP) is the cargo and it's attached to the targeting peptide to be sequestered within the encapsulin. This serves to protect the bacterial host from the AMP that it's producing. The TEV protease site attaches the targeting peptide and AMP so the AMP can be isolated later on in downstream purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 191
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]