Difference between revisions of "Part:BBa K4226004"
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<partinfo>BBa_K4226004 short</partinfo>
 
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The Amp30E Amplification Device is an amplifier module includes ([[Part:BBa K4226001|hrpR Gene]]) , hrpS (BBa_K4226002) and hrpL Promoter (BBa_K4226003),this engineered multi-layered transcriptional amplifiers can be able to sequentially boost output expression level of target genes.
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The Amp30E Amplification Device is an amplifier module includes [[Part:BBa K4226001|hrpR Gene]], Part:BBa K4226002|hrpS Gene]] and [[Part:BBa K4226003|hrpL Promoter]], this engineered multi-layered transcriptional amplifiers can be able to sequentially boost output expression level of target genes.
  
  

Revision as of 05:23, 27 September 2022


Amp30E Amplification Device

The Amp30E Amplification Device is an amplifier module includes hrpR Gene, Part:BBa K4226002|hrpS Gene]] and hrpL Promoter, this engineered multi-layered transcriptional amplifiers can be able to sequentially boost output expression level of target genes.


Usage and Biology

Characterized by CAFA_China 2022


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1891
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101
    Illegal SapI.rc site found at 1734



Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.