Difference between revisions of "Part:BBa K4226002"

Revision as of 10:29, 29 September 2022

hrpS Gene

This part is an improvement for the part：HrpS Gene uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of hrpL promoter (hrpL Promoter) . The Amp30E Amplification Device consists of hrpR (hrpR Gene), hrpS and hrpL promoter (hrpL Promoter) .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 902
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 112
Illegal SapI.rc site found at 745

Reference
[1] Wan, Xinyi, et al. "Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals." Nature chemical biology 15.5 (2019): 540-548.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4226002 short</partinfo>
-This part is an improvement for the part：[[BBa_K1014000|HrpS Gene]] uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of hrpL promoter ([[Part:BBa K4226003|hrpL Promoter]]) . The Amp30E Amplification Device consists of hrpR ([[Part:BBa K4226001|hrpR Gene]]), hrpS and hrpL promoter ([[Part:BBa K4226003|hrpL Promoter]]) .
+This part is an improvement for the part：[[BBa_K1014000|HrpS Gene]] uploaded by iGEM13_HIT-Harbin. The hrpS Gene codes for hrpS protein. The hrpR protein binds to hrpS protein forming a complex, causing a transcriptionally upregulated of hrpL promoter ([[Part:BBa K4226003|hrpL Promoter]]) . The [[Part:BBa_K4226004|Amp30E Amplification Device]] consists of hrpR ([[Part:BBa K4226001|hrpR Gene]]), hrpS and hrpL promoter ([[Part:BBa K4226003|hrpL Promoter]]) .
 <!-- Add more about the biology of this part here