Difference between revisions of "Part:BBa K4421030"

Revision as of 06:58, 16 July 2022

pSV40-UAS-iCASP9-2A-GFP

An inducible caspase-9 suicide gene was placed under the control of a combined UAS-SV40 promoter. The GFP tag serves as a reporter and the 2A peptide sequence was intercalated between iCASP9 and the GFP tag to allow the fusion peptide chain to separate into two peptide chains.

pSV40-UAS-iCASP9-2A-GFP

The mechanism and usage of pSV40-UAS-iCASP9-2A-GFP

This part is placed downstream of NFAT_RE-Gal4-KRAB(BBa_K4421029) and the presence of Gal4-KRAB can inhibit the promotor pSV40-UAS(BBa_K4040003), thus diminishing the synthesis of the iCASP9(BBa_K4421002).The engineered cells can survive if the concentration of iCASP9 is low enough after AP1903 addition.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 781
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 463
Illegal BsaI.rc site found at 1603
Illegal BsaI.rc site found at 2514

@@ Line 13: / Line 13: @@
 ===The mechanism and usage of pSV40-UAS-iCASP9-2A-GFP===
-This part is placed downstream of NFAT_RE-Gal4-KRAB(<partinfo>BBa_K4421029</partinfo>) and the presence of Gal4-KRAB can inhibit the promotor pSV40-UAS, thus diminishing the synthesis of the iCASP9.The engineered cells can survive if the concentration of iCASP9(<partinfo>BBa_K4421002</partinfo>) is low enough after AP1903 addition.
+This part is placed downstream of NFAT_RE-Gal4-KRAB(<partinfo>BBa_K4421029</partinfo>) and the presence of Gal4-KRAB can inhibit the promotor pSV40-UAS(<partinfo>BBa_K4040003</partinfo>), thus diminishing the synthesis of the iCASP9(<partinfo>BBa_K4421002</partinfo>).The engineered cells can survive if the concentration of iCASP9 is low enough after AP1903 addition.
 <!-- Add more about the biology of this part here