Difference between revisions of "Part:BBa K3861017"
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<partinfo>BBa_K3861017 short</partinfo>
 
<partinfo>BBa_K3861017 short</partinfo>
  
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==='''Introduction'''===
 
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<html>
 
Composite part containing the PlldR promoter (<a href="https://parts.igem.org/Part:BBa_K1847008">BBa_K1847008</a>) fused to GFP (<a href="https://parts.igem.org/Part:BBa_E0840">BBa_E0840</a>) as a reporter gene. Both parts were already characterized in depth by other teams. We used this part in <i>Salmonella</i> Typhimurium.
 
Composite part containing the PlldR promoter (<a href="https://parts.igem.org/Part:BBa_K1847008">BBa_K1847008</a>) fused to GFP (<a href="https://parts.igem.org/Part:BBa_E0840">BBa_E0840</a>) as a reporter gene. Both parts were already characterized in depth by other teams. We used this part in <i>Salmonella</i> Typhimurium.
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<partinfo>BBa_K3861017 parameters</partinfo>
 
<partinfo>BBa_K3861017 parameters</partinfo>
 
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==='''Characterization'''===
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<figure>
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<img src="https://2021.igem.org/File:T--Humboldt_Berlin--plldR_characterisation.png" alt="pKF01" width="500">
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<figcaption>Fig. 1 - GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).</figcaption>
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</figure>
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Revision as of 18:46, 21 October 2021


PlldR-GFP

Introduction

Composite part containing the PlldR promoter (BBa_K1847008) fused to GFP (BBa_E0840) as a reporter gene. Both parts were already characterized in depth by other teams. We used this part in Salmonella Typhimurium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 816


Characterization

pKF01
Fig. 1 - GFP expression in pSB1C3 (plate on top) and in pKF01 (plate below).