Difference between revisions of "Part:BBa K3889012"
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<p><b>Parameters used:</b></p> | <p><b>Parameters used:</b></p> | ||
| − | <p>Rate of P22 production = 9218880/day</p> | + | <p>Rate of P22 production = 9218880/nM*day</p> |
<p>Rate of P22 degradation = 1.69/day</p> | <p>Rate of P22 degradation = 1.69/day</p> | ||
</body> | </body> | ||
Revision as of 02:29, 17 October 2021
Bacillus subtilis Synthetic Promoter 200
This is a synthetically derived promoter sequence for the expression of genes in Bacillus subtilis.
|
Promoter |
Sequence 5‘ → 3‘ |
Relative activity wrt P43 of GFP (%) |
Standard deviation |
|
SP126 |
AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT |
29.07 |
0.23 |
|
SP146 |
AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT |
40.39 |
0.69 |
|
SP200 |
AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA |
76.82 |
0.9 |
Usage and Biology
SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are characterised to have constitutive expression, hence they can be used in general protein production. Since the library contains 214 synthetic promoters providing variability and robustness in expression, according to the system's need. All SP promoters have the following consensus sequences:
Based on this the promoters are as follows:
Modelling
We planned to use SP200 with BBa_K1351031 for gene expression of mf-Lon (BBa_K3889101).
Parameters used:
Rate of P22 production = 9218880/nM*day
Rate of P22 degradation = 1.69/day
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]