Difference between revisions of "Part:BBa K4040023"
(→‎Usage and Biology)
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<partinfo>BBa_K4040023 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4040023 SequenceAndFeatures</partinfo>
 
===Usage and Biology===
 
===Usage and Biology===
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The composite part consists of seven parts: the promoter UAS-PSV40 (<partinfo>BBa_K4040033</partinfo>), GFP Genera (<partinfo>E0840</partinfo>), which initiates the expression of coding sequence. pCMV(<partinfo>BBa_I712004</partinfo>) was used as the promoter. The indicator which detects the expression of the pathway and the expression component of CAR-FcRγ: CD8a signal peptide (<partinfo>BBa_K4040014</partinfo>), CR3022 scFv (<partinfo>BBa_K4040019</partinfo>), CD8 Hinge (<partinfo>BBa_K4040004</partinfo>), CD8TM (<partinfo>BBa_K4040007</partinfo>), FcRγ (<partinfo>BBa_K4040002</partinfo>).  When the GAL4-KRAb fragment from IL-6 complex receptor <partinfo>BBa_K4040021</partinfo> is released, it binds to the promoter UAS-PSV40 to inhibit the expression of CAR- FcRγ and reduce the transformation of macrophages into M1 state to alleviate the inflammatory response.  
+
The composite part consists of two parts: the promoter UAS-PSV40 (<partinfo>BBa_K4040033</partinfo>), GFP Genera (<partinfo>E0840</partinfo>), which initiates the expression of coding sequence. When the GAL4-KRAB fragment from IL-6 complex receptor <partinfo>BBa_K4040021</partinfo> is released, it binds to the promoter UAS-PSV40 to inhibit the expression of CAR- FcRγ and reduce the transformation of macrophages into M1 state to alleviate the inflammatory response.  
 
[[File:T--NMU China--Expression of CARγ.jpg|600px|thumb|center|<b>Figure 1.</b>The coding of the CAR-gamma.]]
 
[[File:T--NMU China--Expression of CARγ.jpg|600px|thumb|center|<b>Figure 1.</b>The coding of the CAR-gamma.]]
 
[[File:T--NMU China--IL-6R.jpg|550px|thumb|center|<b>Figure 2.</b>The structure and downstream pathway of the composite IL6R.]]
 
[[File:T--NMU China--IL-6R.jpg|550px|thumb|center|<b>Figure 2.</b>The structure and downstream pathway of the composite IL6R.]]

Revision as of 13:18, 15 October 2021


pSV40-UAS-GFP Expression Cassette

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1115

Usage and Biology

The composite part consists of two parts: the promoter UAS-PSV40 (BBa_K4040033), GFP Genera (BBa_E0840), which initiates the expression of coding sequence. When the GAL4-KRAB fragment from IL-6 complex receptor BBa_K4040021 is released, it binds to the promoter UAS-PSV40 to inhibit the expression of CAR- FcRγ and reduce the transformation of macrophages into M1 state to alleviate the inflammatory response.

Figure 1.The coding of the CAR-gamma.
Figure 2.The structure and downstream pathway of the composite IL6R.