Difference between revisions of "Part:BBa K3889010"
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SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need. All SP promoters have the following consensus sequences:
 
SP126(<partinfo>BBa_K3889010</partinfo>), SP146(<partinfo>BBa_K3889011</partinfo>), SP200(<partinfo>BBa_K3889010</partinfo>) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need. All SP promoters have the following consensus sequences:
  
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[[File:T--IISER-Tirupati India--SP backbone.jpg|900px|left]]
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[[File:T--IISER-Tirupati India--SP backbone.jpeg|900px|left]]
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:58, 9 October 2021


Bacillus Subtilis Synthetic Promoter 126

This is a synthetically derived promoter sequence for the expression of genes in Bacillus subtilis.

Promotor

Sequence 5‘ 3‘

Relative

activity wrt P43 of

GFP (%)

Standard

deviation

SP126

AAAAATTATAAAAATGTGTTGACAAAGGGGGTCCTGTATGTTATAATAGCTT

29.07

0.23

SP146

AAAAATAACAAAAACGTGTTGACAATAAAGATTAACCGTGATATAATTAAAT

40.39

0.69

SP200

AAAAATTAGAAAAATGTGTTGACACTCGGACGAAACAATGGTATAATGGCAA

76.82

0.9
Synthetic Promoters data from SP1-214[1]


Usage and Biology

SP126(BBa_K3889010), SP146(BBa_K3889011), SP200(BBa_K3889010) are a part of synthetic library promoters from Liu et al. (2018)[1]. These synthetic promoters are constitutive hence can be used in general protein production. Since the library contains 214 synthetic promoters providing with variability in expression according to the system need. All SP promoters have the following consensus sequences:

T--IISER-Tirupati India--SP backbone.jpeg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Liu, D., Mao, Z., Guo, J., Wei, L., Ma, H., Tang, Y., Chen, T., Wang, Z., & Zhao, X. (2018). Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis. ACS Synthetic Biology, 7(7), 1785–1797. https://doi.org/10.1021/acssynbio.8b00115