Difference between revisions of "Part:BBa K4047003"
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===Long Description=== | ===Long Description=== | ||
− | This is the reverse primer to amplify the gene for fructose-1,6-bisphosphatase. The overlap sequence | + | This is the reverse primer to amplify the gene for fructose-1,6-bisphosphatase (BBa_K4047035). The overlap sequence allows proper Gibson assembly with the pGEM-fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification. |
− | This is also the reverse primer to amplify the gene for fructose-1,6-bisphosphatase prior to the assembly of pGEM-tal+fbp. The overlap sequence | + | This is also the reverse primer to amplify the gene for fructose-1,6-bisphosphatase prior to the assembly of pGEM-tal+fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone, the same used for pGEM-fbp. The annealing portion (all caps) allows annealing to the wild type S. elongatus PCC 7942 genome for proper amplification. |
Latest revision as of 04:25, 16 October 2021
primerF/TF_fbp-reverse
Reverse primer for FBPase
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 20
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 1000COMPATIBLE WITH RFC[1000]
Long Description
This is the reverse primer to amplify the gene for fructose-1,6-bisphosphatase (BBa_K4047035). The overlap sequence allows proper Gibson assembly with the pGEM-fbp backbone (BBa_K4047024). The annealing portion (all caps) allows annealing to the wild type Synechococcus elongatus PCC 7942 genome for proper amplification.
This is also the reverse primer to amplify the gene for fructose-1,6-bisphosphatase prior to the assembly of pGEM-tal+fbp (BBa_K4047038). The overlap sequence allows proper Gibson assembly with the pGEM-tal+fbp backbone, the same used for pGEM-fbp. The annealing portion (all caps) allows annealing to the wild type S. elongatus PCC 7942 genome for proper amplification.