Difference between revisions of "Part:BBa K4047003"
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This is the reverse primer to amplify the gene for fructose-1,6-bisphosphatase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. | This is the reverse primer to amplify the gene for fructose-1,6-bisphosphatase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. | ||
This is also the reverse primer to amplify the gene for fructose-1,6-bisphosphatase prior to the assembly of pGEM-tal+fbp. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal+fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. | This is also the reverse primer to amplify the gene for fructose-1,6-bisphosphatase prior to the assembly of pGEM-tal+fbp. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal+fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. | ||
Revision as of 18:10, 4 October 2021
primerF/TF_fbp-reverse
Reverse primer for FBPase
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 20
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 14
Illegal PstI site found at 2 - 1000COMPATIBLE WITH RFC[1000]
Long Description
This is the reverse primer to amplify the gene for fructose-1,6-bisphosphatase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.
This is also the reverse primer to amplify the gene for fructose-1,6-bisphosphatase prior to the assembly of pGEM-tal+fbp. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal+fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.