Difference between revisions of "Part:BBa K4047002"
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<partinfo>BBa_K4047002 short</partinfo>
 
<partinfo>BBa_K4047002 short</partinfo>
  
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Reverse primer for FBPase
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:57, 4 October 2021


primerF_fbp-foward


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


This is the forward primer to amplify the gene for fructose-1,6-bisphosphatase class 1 (Synpcc7942_2335). The overlap sequence (red) allows proper Gibson assembly with the pGEM-fbp backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.