Difference between revisions of "Part:BBa K4047002:Design"
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===Source=== | ===Source=== | ||
− | + | Generated from a combination of factors. | |
− | + | Overlap sequence sourced from pAM2991. | |
− | + | https://www.addgene.org/40248/ | |
+ | Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. | ||
+ | https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2 | ||
===References=== | ===References=== |
Revision as of 17:58, 4 October 2021
primerF_fbp-foward
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 20
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 20
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 20
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 20
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 20
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
Generated from a combination of factors. Overlap sequence sourced from pAM2991. https://www.addgene.org/40248/ Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for fructose-1,6-bisphosphatase class I, at the locus tag Synpcc7942_2335. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2402921&to=2403955&report=fasta&strand=2