Difference between revisions of "Part:BBa K4047001"
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− | + | This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification. | |
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Revision as of 17:53, 4 October 2021
This is the reverse primer to amplify the gene for transaldolase. The overlap sequence (red) allows proper Gibson assembly with the pGEM-tal backbone. The annealing portion (all caps) allows annealing to the WT S. elongatus PCC 7942 genome for proper amplification.