Difference between revisions of "Part:BBa K165018"
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Built in the Silver Lab [http://2008.igem.org/Team:BrownTwo/Implementation/syntf Biofusion]standard. | Built in the Silver Lab [http://2008.igem.org/Team:BrownTwo/Implementation/syntf Biofusion]standard. | ||
| − | The SV40 nuclear localization sequence can be added to any coding region to localize its expression to the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences. | + | The SV40 nuclear localization sequence codes for a series of amino acids that tag a protein to be transported into the nucleus in eukaryotes.[http://www.ncbi.nlm.nih.gov/pubmed/6096007] It can be added to any coding region to localize its expression to the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences. |
To build a transcriptional repressor, ligate a DNA-binding domain to this NLS+terminaor. Add the resulting part downstream of [https://parts.igem.org/Part:BBa_K165028 Kozak+Sin3 Repression Domain] | To build a transcriptional repressor, ligate a DNA-binding domain to this NLS+terminaor. Add the resulting part downstream of [https://parts.igem.org/Part:BBa_K165028 Kozak+Sin3 Repression Domain] | ||
Latest revision as of 19:10, 2 November 2008
SV40 nuclear localization sequence + ADH1 transcription terminator
Built in the Silver Lab [http://2008.igem.org/Team:BrownTwo/Implementation/syntf Biofusion]standard.
The SV40 nuclear localization sequence codes for a series of amino acids that tag a protein to be transported into the nucleus in eukaryotes.[http://www.ncbi.nlm.nih.gov/pubmed/6096007] It can be added to any coding region to localize its expression to the nucleus in yeast. This is useful for building synthetic transcription factors. In this part, it is ligated to the ADH1 transcription terminator for convenient cloning to the end of coding sequences.
To build a transcriptional repressor, ligate a DNA-binding domain to this NLS+terminaor. Add the resulting part downstream of Kozak+Sin3 Repression Domain
The LexA repressor has also been shown to work with the Sin3 repression domain and DNA-binding domain switched, in this order:
Kozak + LexA DBD + Sin3 + NLS + Term
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]