Difference between revisions of "Part:BBa K3425054"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <span class='h3bb'>Sequence and Features</span> | ||
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Latest revision as of 00:16, 28 October 2020
Constitutive expression construct of Juniper GFP
Asembled using Type IIS iGEM Loop assembly standard from the constitutive promoter, BBa_J23100, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425031, the Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the double terminator BBa_B0015.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 476
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 476
Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 476
Illegal BamHI site found at 685 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 476
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 476
- 1000COMPATIBLE WITH RFC[1000]