Difference between revisions of "Part:BBa K3411040"
| Line 9: | Line 9: | ||
The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, [https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018]) system serves as an acceptor of the phosphate group from NarX. The promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, initiates the production of TetR. TetR then represses the PtetO promoter. By regulating the amount of aTc, the molecule that inhibits TetR, the team can then control levels of NarL. In the presence of nitrate, NarX phosphorylates NarL. Then, phosphorylated NarL binds to a specified promoter and correlates to measured nitrate levels. Further details about the continued expression of the NarL part are listed in the description HKUST-Rice provides on their part. Therefore, phosphorylated NarL measures nitrate levels. | The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, [https://parts.igem.org/Part:BBa_K1682018 BBa_K1682018]) system serves as an acceptor of the phosphate group from NarX. The promoter [https://parts.igem.org/Part:BBa_J23106 BBa_J23106], a constitutive Anderson Promoter, initiates the production of TetR. TetR then represses the PtetO promoter. By regulating the amount of aTc, the molecule that inhibits TetR, the team can then control levels of NarL. In the presence of nitrate, NarX phosphorylates NarL. Then, phosphorylated NarL binds to a specified promoter and correlates to measured nitrate levels. Further details about the continued expression of the NarL part are listed in the description HKUST-Rice provides on their part. Therefore, phosphorylated NarL measures nitrate levels. | ||
| − | [[Image:NARLDESIGN.png|500px|thumb|center| | + | [[Image:NARLDESIGN.png|500px|thumb|center|Figure 2: Diagram of the nitrate/nitrite detection NarP system construct.]] |
Lambert_GA iGEM 2020 will continue cloning and begin characterization of our NarL part in the coming year as a part of our two-year project, AgroSense. | Lambert_GA iGEM 2020 will continue cloning and begin characterization of our NarL part in the coming year as a part of our two-year project, AgroSense. | ||
Revision as of 02:23, 28 October 2020
NarL Gene
Lambert_GA iGEM 2020's NarL system is modeled after E.coli’s natural nitrate sensor, the Nar Operon, which includes the NarL response regulator, and NarX, the membrane-bound sensor protein. The NarL/NarX system detects nitrate. This specific part is the isolated NarL gene.
Description
The listed NarL’s role within Lambert_GA iGEM 2020’s (improved from HKUST-Rice 2015, BBa_K1682018) system serves as an acceptor of the phosphate group from NarX. The promoter BBa_J23106, a constitutive Anderson Promoter, initiates the production of TetR. TetR then represses the PtetO promoter. By regulating the amount of aTc, the molecule that inhibits TetR, the team can then control levels of NarL. In the presence of nitrate, NarX phosphorylates NarL. Then, phosphorylated NarL binds to a specified promoter and correlates to measured nitrate levels. Further details about the continued expression of the NarL part are listed in the description HKUST-Rice provides on their part. Therefore, phosphorylated NarL measures nitrate levels.
Lambert_GA iGEM 2020 will continue cloning and begin characterization of our NarL part in the coming year as a part of our two-year project, AgroSense.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 174
Illegal BglII site found at 342 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]