Difference between revisions of "Part:BBa K3505044"
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<partinfo>BBa_K3505044 short</partinfo> | <partinfo>BBa_K3505044 short</partinfo> | ||
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eCFP <bbpart>BBa_K3505020</bbpart>uder control of a constitutive promoter (andersonJ23115 with a tet operator) <bbpart>BBa_K2924014</bbpart>. | eCFP <bbpart>BBa_K3505020</bbpart>uder control of a constitutive promoter (andersonJ23115 with a tet operator) <bbpart>BBa_K2924014</bbpart>. | ||
Revision as of 07:18, 27 October 2020
pAndersonJ23115:TetO:RBS-eCFP -terminator
eCFP BBa_K3505020uder control of a constitutive promoter (andersonJ23115 with a tet operator) BBa_K2924014.
Usage and Biology
This Trancriscription Unit (TU) is continuesly activated exressing the eCFP protein as a reporter. The Tet operator that is downsteam the anderson exists for the TetR regulated inhibition.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in a1R BBa_K3505008 and has overhangs compatible for Golden Braid cloning.
Verification of cloning
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]