Difference between revisions of "Part:BBa K3505032"
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===Verification of cloning=== | ===Verification of cloning=== | ||
− | [[File:T--Thessaly-LE-digestion.png|600px|thumb|none|<i><b>Fig.2:</b>(U=Uncut C=Cut) (U=Uncut , C= Cut) Restriction digestion of LE: AndersonJ23115:Lac0-EGFP-double terminator(C1-C 4) with : | + | [[File:T--Thessaly-LE-digestion.png|600px|thumb|none|<i><b>Fig.2:</b>(U=Uncut C=Cut) (U=Uncut , C= Cut) Restriction digestion of LE: AndersonJ23115:Lac0-EGFP-double terminator(C1-C 4) with : HindIII + BtgZI (C1-C4) , Expected bands : 3200+ 597bp ,Positive result: C1,C2</i>]] |
===Experimental Use and Experinece=== | ===Experimental Use and Experinece=== |
Revision as of 13:46, 26 October 2020
pAndersonJ23115:lacO:RBS-eCFP -terminator
eCFP BBa_K3505020uder control of a constitutive promoter (andersonJ23115 with a lac operator) BBa_K2924013.
Usage and Biology
This Trancriscription Unit (TU) is continuesly activated exressing the eCFP protein as a reporter. The Lac operator that is downsteam the anderson exists for the lac regulated inhibition.
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in both a1R BBa_K3505008and a2 BBa_K3505009 and has overhangs compatible for Golden Braid cloning.
Verification of cloning
Experimental Use and Experinece
This part is used in BBa_K3505036
Extra Engineering Use
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]