Difference between revisions of "Part:BBa K3505014"
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Constitutive Anderson <bbpart>BBa_J23115</bbpart>with <i>Tet Operator</i> and RBS <bbpart>BBa_B0034</bbpart> | Constitutive Anderson <bbpart>BBa_J23115</bbpart>with <i>Tet Operator</i> and RBS <bbpart>BBa_B0034</bbpart> | ||
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The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning. | The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for Golden Braid cloning. | ||
This has position A1-B3. | This has position A1-B3. | ||
− | [[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig. | + | [[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the Golden Braid Grammar</i>]] |
− | + | [[Image:T--Thessaly--andersontetO-snap.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- AndersonJ23115:TetO:RBS (illustration from SnapGene)</i>]] | |
+ | ===Verification of cloning=== | ||
+ | [[File:T--Thessaly--TET0-digestion.png |700px|thumb|none|<i><b>Fig.1:</b>Figure 7: (U=Uncut , C= Cut) Restriction digestion of TetO (C1-C4 ) with :Not-HF(C1-C4) , Expected bands : 2046 + 129 bp, Positive result: C1,C2,C4</i>]] | ||
===Experimental Use and Experience=== | ===Experimental Use and Experience=== |
Revision as of 11:21, 26 October 2020
AndersonJ23115:TetO:RBS GB compatible with A1-B2
Constitutive Anderson BBa_J23115with Tet Operator and RBS BBa_B0034
Design Notes
The sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for Golden Braid cloning. This has position A1-B3.
Verification of cloning
Experimental Use and Experience
This part is used in BBa_K3505034
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 11
Illegal NheI site found at 34 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]