Difference between revisions of "Promoters/Catalog"
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The registry has a large collection of [[Promoters]] available.  We categorize promoters using two different parameters.  First, most promoters are designed for a particular RNA polymerase (RNAP) holoenzyme, e.g ''E. coli'' RNAP with a particular sigma factor (for example σ70) that confers specificity on the RNAP holoenzyme , or the RNAP from the T7 bacteriophage.  Second, promoters are categorized by the manner in which they are regulated.  For example a promoter may be unregulated (constitutive), positively regulated (inducible) or negatively regulated (repressible).  Regulated promoters allow you to "program" biological systems.  For example, you can perform logic (e.g. "If protein X is present, promoter Y is off") or ensure that a particular gene is only expressed when a particular small molecule is present in the media.
+
The registry has a large collection of [[Promoters]] available.  We categorize promoters in two ways.  First, most promoters are designed for a particular RNA polymerase (RNAP) holoenzyme, e.g ''E. coli'' RNAP bound to σ70 (σ70 is a sigma factor, one of a group of proteins that confers specificity on ''E. coli'' RNAP for particular promoter sequences), or the RNAP from the T7 bacteriophage.  Second, promoters are categorized by the manner in which they are regulated.  For example a promoter may be unregulated (constitutive), positively regulated (activatable) or negatively regulated (repressible).  Regulated promoters allow you to "program" biological systems.  For example, you can perform logic (e.g. "If protein X is present, promoter Y is off") or ensure that a particular gene is only expressed when a particular small molecule is present in the media.
  
 
The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.
 
The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.
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{|align="center" border="1" cellpadding="0" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;" width="100%"
 
{|align="center" border="1" cellpadding="0" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;" width="100%"
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!rowspan="3" width="120px"|Regulation
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!rowspan="4" width="120px"|Regulation
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!colspan="3"|RNA Polymerase
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!colspan="11"|RNA Polymerase
 
|-
 
|-
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|align="center" colspan="2"|[[Help:Promoters/E.coli RNAP|''E. coli'']]
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|align="center" colspan="7"|[[Help:Promoters/Prokaryotic RNAP|''Prokaryotic'']]
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|align="center"|[[Help:Promoters/T7RNAP|T7]]
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|align="center" colspan="2"|[[Help:Promoters/Bacteriophage RNAP|Bacteriophage]]
 +
|align="center" colspan="2"|[[Help:Promoters/Eukaryotic RNAP|Eukaryotic]]
 +
|-
 +
|align="center" colspan="4"|[[Help:Promoters/E. coli RNAP|''E. coli'']]
 +
|align="center" colspan="2"|[[Help:Promoters/B. subtilis RNAP|''B. subtilis'']]
 +
|align="center" colspan="1"|[[Help:Promoters/E. coli RNAP|''Synechocystis sp'']]
 +
|align="center"|[[Help:Promoters/T7 RNAP|T7]]
 +
|align="center"|[[Help:Promoters/SP6 RNAP|SP6]]
 +
|align="center"|[[Help:Promoters/Eukaryotic RNAP|General]]
 +
|align="center"|[[Help:Promoters/Yeast RNAP|Yeast]]
 
|-
 
|-
 
|align="center"|[[Help:Promoters/Sigma70|&sigma;<sup>70</sup>]]
 
|align="center"|[[Help:Promoters/Sigma70|&sigma;<sup>70</sup>]]
 
|align="center"|[[Help:Promoters/SigmaS|&sigma;<sup>S</sup>]]
 
|align="center"|[[Help:Promoters/SigmaS|&sigma;<sup>S</sup>]]
 +
|align="center"|[[Help:Promoters/Sigma32|&sigma;<sup>32</sup>]]
 +
|align="center"|[[Help:Promoters/Sigma54|&sigma;<sup>54</sup>]]
 +
|align="center"|[[Help:Promoters/SigmaA|&sigma;<sup>A</sup>]]
 +
|align="center"|[[Help:Promoters/SigmaB|&sigma;<sup>B</sup>]]
 +
|
 +
|
 +
|
 +
|
 
|
 
|
 
|-style="height:58px"  
 
|-style="height:58px"  
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|[[Image:BCMultipleIcon.png]]
 
|[[Image:BCMultipleIcon.png]]
 
|}
 
|}
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|align="center"| See <count_promoters inducible sigma70></count_promoters> promoters
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|align="center"|210 promoters
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|align="center"| See X promoters
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|align="center"|210 promoters
−
|align="center"| See X promoters
+
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 
|-style="height:58px"  
 
|-style="height:58px"  
−
|align="center"| See X promoters
+
|align="center"|210 promoters
−
|align="center"| See X promoters
+
|align="center"|210 promoters
−
|align="center"| See X promoters
+
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 
|-style="height:58px"  
 
|-style="height:58px"  
−
|align="center"| See X promoters
+
|align="center"|210 promoters
−
|align="center"| See X promoters
+
|align="center"|210 promoters
−
|align="center"| See X promoters
+
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 
|-style="height:38px"  
 
|-style="height:38px"  
−
|align="center"| See X promoters
+
|align="center"|210 promoters
−
|align="center"| See X promoters
+
|align="center"|210 promoters
−
|align="center"| See X promoters
+
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 +
|align="center"|210 promoters
 
|-
 
|-
 
|}
 
|}

Revision as of 04:13, 29 October 2008

The registry has a large collection of Promoters available. We categorize promoters in two ways. First, most promoters are designed for a particular RNA polymerase (RNAP) holoenzyme, e.g E. coli RNAP bound to σ70 (σ70 is a sigma factor, one of a group of proteins that confers specificity on E. coli RNAP for particular promoter sequences), or the RNAP from the T7 bacteriophage. Second, promoters are categorized by the manner in which they are regulated. For example a promoter may be unregulated (constitutive), positively regulated (activatable) or negatively regulated (repressible). Regulated promoters allow you to "program" biological systems. For example, you can perform logic (e.g. "If protein X is present, promoter Y is off") or ensure that a particular gene is only expressed when a particular small molecule is present in the media.

The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.


Regulation RNA Polymerase
Prokaryotic Bacteriophage Eukaryotic
E. coli B. subtilis Synechocystis sp T7 SP6 General Yeast
σ70 σS σ32 σ54 σA σB
Positive BCInducibleIcon.png
Constitutive ConstitutiveIcon.png
Negative BCRepressibleIcon.png
Multiple BCMultipleIcon.png
210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters
210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters
210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters
210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters 210 promoters