Difference between revisions of "Part:BBa K103019:Experience"
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*protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_alpha_Z | *protein Z<sub>SPA-1</sub> [[Part:BBa_K103004 | BBa_K103004]]. Resulting fusion was named His_alpha_Z | ||
| − | Both fusions were overexpressed in | + | Both fusions were overexpressed in Roseta strain. Due to ability of protein Z to form aggregates His_alpha_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. |
His_alpha_A was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha purified] on NiNTA-agarose bead (elution peak in 150 mM imidazole). | His_alpha_A was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha purified] on NiNTA-agarose bead (elution peak in 150 mM imidazole). | ||
The both prey protein fusions were added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]]. | The both prey protein fusions were added to culture media and used in selection experiments. Details [[Part:BBa_K103017:Experience | here]] and [[Part:BBa_K103018:Experience | here]]. | ||
Latest revision as of 17:56, 28 October 2008
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how you used this part and how it worked out.
Applications of BBa_K103019
'Hunter and prey' selection system
This part was used as core of prey generator device. It was fused with:
- protein A BBa_K103003. Resulting fusion was named His_alpha_A
- protein ZSPA-1 BBa_K103004. Resulting fusion was named His_alpha_Z
Both fusions were overexpressed in Roseta strain. Due to ability of protein Z to form aggregates His_alpha_Z was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=9&arg0=28_July_2008&arg1=29_July_2008&arg2=30_July_2008&arg3=31_July_2008&arg4=7_August_2008&arg5=11_August_2008&arg6=12_August_2008&arg7=14_August_2008&arg8=18_August_2008&name=Purification%20of%20proteins%3A%20Z-alpha%20and%20Z-omega purified] simply by collecting post-sonication debris. His_alpha_A was [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=8&arg0=29_August_2008&arg1=1_September_2008&arg2=2_September_2008&arg3=3_September_2008&arg4=4_September_2008&arg5=5_September_2008&arg6=8_September_2008&arg7=9_September_2008&name=Purification%20of%20proteins%3A%20A-alpha purified] on NiNTA-agarose bead (elution peak in 150 mM imidazole). The both prey protein fusions were added to culture media and used in selection experiments. Details here and here.
User Reviews
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