Difference between revisions of "Part:BBa K3610003"

Revision as of 18:26, 30 September 2020

BAK1 Ectodomain from Arabidopsis thaliana

This part is a version of the BAK1 receptor (Part:BBa_K3610001 for full length) without the intracellular kinase domain and without the native signal sequence.

Usage and Biology

The BAK1 cell surface receptor from A. thaliana consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a kinase domain and initiates a signal cascade upon dimerization with a plant PRR to activate the plant immune response.

For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output. In our iGEM project, we fused this sequence to a split-mCherry protein and a split-luciferase and then coexpressed the part with another plant PRR carrying the counterpart to the split protein instead of the intracellular kinase domain.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 576
Illegal PstI site found at 621
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 576
Illegal PstI site found at 621
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 576
Illegal PstI site found at 621
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 576
Illegal PstI site found at 621
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3610003 short</partinfo>
-The BAK1 cell surface receptor from A. thaliana [[Part:BBa_K3610004]] consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a protein kinase.
+This part is a version of the BAK1 receptor ([[Part:BBa_K3610001]] for full length) without the intracellular kinase domain and without the native signal sequence.
-For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output.
-This part has the is the BAK1 receptor without the kinase domain and without the native signal sequence.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The BAK1 cell surface receptor from A. thaliana consists of a single pass transmembrane domain and two topoligical domains, an extracellular and a cytoplasmic one. The cytoplasmic domain functions as a kinase domain and initiates a signal cascade upon dimerization with a plant PRR to activate the plant immune response.
+For visualizing the interaction of BAK1 with other plant receptors the cytoplasmic domain can be replaced with a split fluorescent protein or another protein that generates a visual output.
+In our iGEM project, we fused this sequence to a split-mCherry protein and a split-luciferase and then coexpressed the part with another plant PRR carrying the counterpart to the split protein instead of the intracellular kinase domain.
 <!-- -->