Difference between revisions of "Part:BBa K3037006"
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Made by the TU Dresden 2019 adapted to the RFC 25 standard [https://2019.igem.org/Team:TU_Dresden/Parts (more information).]
 
Made by the TU Dresden 2019 adapted to the RFC 25 standard [https://2019.igem.org/Team:TU_Dresden/Parts (more information).]
 +
 +
== Biology ==
 +
 +
Traditionally isolated from the jellyfish Aequorea victoria, the green fluorescent protein (GFP)
 +
has become an important marker of gene expression [1]. However, due to relatively lower
 +
sensitivity of wild-type GFP compared to standard reporter proteins, many different mutants of
 +
GFP have been engineered.
 +
Thereby, mutations introduced in chromophore of GFP significantly improved its detection and
 +
made the protein 35 times brighter than wild-type GFP. The modified fluorescent protein, also
 +
known as enhanced GFP (eGFP), appeared to be more sensitive compared to initial detection
 +
results [2].
  
 
== Sequence ==
 
== Sequence ==
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<partinfo>BBa_K3037006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3037006 SequenceAndFeatures</partinfo>
  
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== References ==
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[1] Stepanenko OV, Verkhusha VV, Kuznetsova IM, Uversky VN, Turoverov KK (Aug 2008).
 +
"Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and
 +
cellular processes". Current Protein & Peptide Science. 9 (4): 338–69
 +
 +
[2] Zhang, G., Gurtu, V., & Kain, S. R. (1996). An Enhanced Green Fluorescent Protein Allows
 +
Sensitive Detection of Gene Transfer in Mammalian Cells. Biochemical and Biophysical
 +
Research Communications, 227(3), 707–711.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 16:43, 21 October 2019

eGFP

eGFP
Function Expression, Reporter
Use in Escherichia coli
RFC standard Freiburg RFC25 standard
Backbone pSB1C3
Experimental Backbone pOCC97
Submitted by Team: TU_Dresden 2019



Overview

Made by the TU Dresden 2019 adapted to the RFC 25 standard (more information).

Biology

Traditionally isolated from the jellyfish Aequorea victoria, the green fluorescent protein (GFP) has become an important marker of gene expression [1]. However, due to relatively lower sensitivity of wild-type GFP compared to standard reporter proteins, many different mutants of GFP have been engineered. Thereby, mutations introduced in chromophore of GFP significantly improved its detection and made the protein 35 times brighter than wild-type GFP. The modified fluorescent protein, also known as enhanced GFP (eGFP), appeared to be more sensitive compared to initial detection results [2].

Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1] Stepanenko OV, Verkhusha VV, Kuznetsova IM, Uversky VN, Turoverov KK (Aug 2008). "Fluorescent proteins as biomarkers and biosensors: throwing color lights on molecular and cellular processes". Current Protein & Peptide Science. 9 (4): 338–69

[2] Zhang, G., Gurtu, V., & Kain, S. R. (1996). An Enhanced Green Fluorescent Protein Allows Sensitive Detection of Gene Transfer in Mammalian Cells. Biochemical and Biophysical Research Communications, 227(3), 707–711.