Difference between revisions of "Part:BBa K3187045"
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This part is a generator for the P22 bacteriophage capsid containing the both capsomers coat protein and scaffold protein. This part was used together with <a | This part is a generator for the P22 bacteriophage capsid containing the both capsomers coat protein and scaffold protein. This part was used together with <a | ||
href=" https://parts.igem.org/Part:BBa_K3187044" target="_blank">BBa_K3187044</a> for the dual expression of the P22 capsid with different ratios of LPETGG-tagged coat protein to untagged coat protein. The potential of fine tuning the expression levels of both parts was tested via <a | href=" https://parts.igem.org/Part:BBa_K3187044" target="_blank">BBa_K3187044</a> for the dual expression of the P22 capsid with different ratios of LPETGG-tagged coat protein to untagged coat protein. The potential of fine tuning the expression levels of both parts was tested via <a | ||
href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>. | href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>. | ||
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Revision as of 08:21, 20 October 2019
P22 Bacteriophage Coat and Scaffolding Protein (tetR/tetA promotor)
This part is a generator for the P22 bacteriophage capsid containing the both capsomers coat protein and scaffold protein. This part was used together with BBa_K3187044 for the dual expression of the P22 capsid with different ratios of LPETGG-tagged coat protein to untagged coat protein. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal PstI site found at 1931 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal NheI site found at 1936
Illegal PstI site found at 1931 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal BamHI site found at 1389 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal PstI site found at 1931 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 76
Illegal EcoRI site found at 1395
Illegal PstI site found at 1931
Illegal NgoMIV site found at 1783 - 1000COMPATIBLE WITH RFC[1000]