Difference between revisions of "Part:BBa K3187044"
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This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins. This part was used together with <a | This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins. This part was used together with <a | ||
href=" https://parts.igem.org/Part:BBa_K3187043" target="_blank">BBa_K3187043</a> for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via <a | href=" https://parts.igem.org/Part:BBa_K3187043" target="_blank">BBa_K3187043</a> for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via <a | ||
href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>. | href=" https://parts.igem.org/Part:BBa_K3187011" target="_blank">BBa_K3187011</a>. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 08:13, 20 October 2019
P22 Bacteriophag Coat Protein (T7 promotor)
This part is a generator for the P22 bacteriophage coat protein fused to a LPETGG-tag for Sortase-mediated ligation of GGGG-tagged proteins. This part was used together with BBa_K3187043 for the dual expression of the P22 scaffold protein and the P22 coat protein to generated P22 Virus-like particles, that can be modified on their surface using sortase-mediated ligation. The potential of fine tuning the expression levels of both parts was tested via BBa_K3187011.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1499
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 98
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1499
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1499
- 1000COMPATIBLE WITH RFC[1000]