Difference between revisions of "Part:BBa K3215017"
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<partinfo>BBa_K3215017 short</partinfo> | <partinfo>BBa_K3215017 short</partinfo> | ||
| − | This part is a 50bp homologous sequence that occurs at region -35 of the phnC promoter from E. coli K-12. It can be used together with phnC UTR 5' homology part (<partinfo>BBa_K3215018</partinfo>) to form a construct containing a flanking region that changes the phn Operon promoter region through recombination techniques, removing the original one (e.g. <partinfo>BBa_K3215007</partinfo>). | + | This part is a 50bp homologous sequence that occurs at region -35 of the phnC promoter from E. coli K-12. It can be used together with phnC UTR 5' homology part (<partinfo>BBa_K3215018</partinfo>) to form a construct containing a flanking region that changes the phn Operon promoter region, through recombination techniques, removing the original one (e.g. <partinfo>BBa_K3215007</partinfo>). |
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Latest revision as of 23:46, 19 October 2019
phnC Promoter homology
This part is a 50bp homologous sequence that occurs at region -35 of the phnC promoter from E. coli K-12. It can be used together with phnC UTR 5' homology part (BBa_K3215018) to form a construct containing a flanking region that changes the phn Operon promoter region, through recombination techniques, removing the original one (e.g. BBa_K3215007).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 33
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 33
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 33
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 33
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 33
- 1000COMPATIBLE WITH RFC[1000]